Tsutsumi Y, Kihira T, Tsunoda S, Kamada H, Nakagawa S, Kaneda Y, Kanamori
T, Mayumi T. Molecular design of hybrid tumor necrosis factor-alpha III: polyethylene glycol-modified tumor necrosis factor-alpha
has markedly enhanced antitumor potency due to longer plasma half-life
and higher tumor accumulation. J Pharmacol Exp Ther., 278:1006-1011. 1996.[PubMed]
Tsutsumi Y, Tsunoda S, Kamada H, Kihira T, Nakagawa S, Kaneda Y, Kanamori
T, Mayumi T. Molecular design of hybrid tumour necrosis factor-alpha. II: The molecular size of polyethylene glycol-modified tumour necrosis
factor-alpha affects its anti-tumour potency. Br J Cancer., 74:1090-1095.
1996.[PubMed]
Tsutsumi Y, Tsunoda S, Kaneda Y, Kamada H, Kihira T, Nakagawa S, Yamamoto
Y, Horisawa Y, Mayumi T. In vivo anti-tumor efficacy of polyethylene glycol-modified
tumor necrosis factor-alpha against tumor necrosis factor-resistant tumors.
Jpn J Cancer Res. 87:1078-1085, 1996.[PubMed]
Tsutsumi Y, Tsunoda S, Kamada H, Kihira T, Kaneda Y, Ohsugi Y, Mayumi T.
PEGylation of interleukin-6 effectively increases its thrombopoietic potency.
Thromb Haemost., 77:168-173. 1997.[PubMed]
Kaneda Y, Yamamoto Y, Tsunoda S, Kamada H, Tsutsumi Y, Hirano T, Mayumi
T. Bioconjugation of tumor necrosis factor-alpha with the copolymer of
divinyl ether and maleic anhydride increasing its antitumor potency. Biochem
Biophys Res Commun., 239:160-165. 1997.[PubMed]
Maeda M, Kawasaki K, Mu Y, Kamada H, Tsutsumi Y, Smith TJ, Mayumi T. Amino
acids and peptides. XXXIII. A bifunctional poly(ethylene glycol) hybrid
of laminin-related peptides. Biochem Biophys Res Commun., 248:485-489.
1998.[PubMed]
Kaneda Y, Yamamoto Y, Kamada H, Tsunoda S, Tsutsumi Y, Hirano T, Mayumi
T. Antitumor activity of tumor necrosis factor alpha conjugated with divinyl
ether and maleic anhydride copolymer on solid tumors in mice. Cancer Res.,
58:290-295. 1998.[PubMed]
Tsunoda S, Ishikawa T, Yamamoto Y, Kamada H, Koizumi K, Matsui J, Tsutsumi
Y, Hirano T, Mayumi T. Enhanced antitumor potency of polyethylene glycolylated
tumor necrosis factor-alpha: a novel polymer-conjugation technique with
a reversible amino-protective reagent. J Pharmacol Exp Ther., 290:368-372.
1999.[PubMed]
Nishiyama Y, Yoshikawa T, Kurita K, Hojo K, Kamada H, Tsutsumi Y, Mayumi
T, Kawasaki K. Regioselective conjugation of chitosan with a laminin-related
peptide, Tyr-Ile-Gly-Ser-Arg, and evaluation of its inhibitory effect on
experimental cancer metastasis. Chem Pharm Bull (Tokyo)., 47:451-453. 1999.[PubMed]
Mu Y, Kamada H, Kaneda Y, Yamamoto Y, Kodaira H, Tsunoda S, Tsutsumi Y,
Maeda M, Kawasaki K, Nomizu M, Yamada Y, Mayumi T. Bioconjugation of laminin
peptide YIGSR with poly(styrene co-maleic acid) increases its antimetastatic
effect on lung metastasis of B16-BL6 melanoma cells. Biochem Biophys Res
Commun., 255:75-79. 1999.[PubMed]
Mu Y, Kamada H, Kodaira H, Sato K, Tsutsumi Y, Maeda M, Kawasaki K, Nomizu
M, Yamada Y, Mayumi T. Bioconjugation of laminin-related peptide YIGSR
with polyvinyl pyrrolidone increases its antimetastatic effect due to a
longer plasma half-life. Biochem Biophys Res Commun., 264:763-767. 1999.[PubMed]
Kamada H, Tsutsumi Y, Tsunoda S, Kihira T, Kaneda Y, Yamamoto Y, Nakagawa S, Horisawa
Y, Mayumi T. Molecular design of conjugated tumor necrosis factor-alpha:
synthesis and characteristics of polyvinyl pyrrolidone modified tumor necrosis
factor-alpha. Biochem Biophys Res Commun., 257:448-453. 1999.[PubMed]
Tsunoda S, Kamada H, Yamamoto Y, Ishikawa T, Matsui J, Koizumi K, Kaneda
Y, Tsutsumi Y, Ohsugi Y, Hirano T, Mayumi T. Molecular design of polyvinylpyrrolidone-conjugated
interleukin-6 for enhancement of in vivo thrombopoietic activity in mice.
J Control Release., 68:335-341. 2000.[PubMed]
K
Kamada H, Tsutsumi Y, Kihira T, Tsunoda S, Yamamoto Y, Mayumi T. In vitro
remodeling of tumor vascular endothelial cells using conditioned medium
from various tumor cells and their sensitivity to TNF-alpha. Biochem Biophys
Res Commun., 268:809-813. 2000.[PubMed]
Kamada H, Tsutsumi Y, Yamamoto Y, Kihira T, Kaneda Y, Mu Y, Kodaira H, Tsunoda
SI, Nakagawa S, Mayumi T. Antitumor activity of tumor necrosis factor-alpha
conjugated with polyvinylpyrrolidone on solid tumors in mice. Cancer Res.,
60:6416-6420. 2000.[PubMed]
Hojo K, Maeda M, Mu Y, Kamada H, Tsutsumi Y, Nishiyama Y, Yoshikawa T,
Kurita K, Block LH, Mayumi T, Kawasaki K. Facile synthesis of a chitosan
hybrid of a laminin-related peptide and its antimetastatic effect in mice.
J Pharm Pharmacol., 52:67-73. 2000.[PubMed]
Tsunoda S, Ishikawa T, Watanabe M, Kamada H, Yamamoto Y, Tsutsumi Y, Hirano
T, Mayumi T. Selective enhancement of thrombopoietic activity of PEGylated
interleukin 6 by a simple procedure using a reversible amino-protective
reagent. Br J Haematol., 112:181-188. 2001.[PubMed]
Koizumi K, Tsutsumi Y, Kamada H, Yoshioka Y, Watanabe M, Yamamoto Y, Okamoto
T, Mukai Y, Nakagawa S, Tani Y, Mayumi T. Incorporation of adult organ-derived
endothelial cells into tumor blood vessel. Biochem Biophys Res Commun.,
306:219-24. 2003.[PubMed]
Kamada H, Tsutsumi Y, Sato-Kamada K, Yamamoto Y, Yoshioka Y, Okamoto T, Nakagawa
S, Nagata S, Mayumi T. Synthesis of a poly(vinylpyrrolidone -co-dimethyl
maleic anhydride) co-polymer and its application for renal drug targeting.
Nat Biotechnol., 21:399-404. 2003.[PubMed]
Yuda H, Adachi Y, Taguchi O, Gabazza EC, Hataji O, Fujimoto H, Tamaki S,
Nishikubo K, Fukudome K, D'Alessandro-Gabazza CN, Maruyama J, Izumizaki
M, Iwase M, Homma I, Inoue R, Kamada H, Hayashi T, Kasper M, Lambrecht
BN, Barnes PJ, Suzuki K. Activated protein C inhibits bronchial hyperresponsiveness
and Th2 cytokine expression in mice. Blood., 103: 2196-2204. 2004.[PubMed]
Yoshioka Y, Tsutsumi Y, Kamada H, Kihira T, Tsunoda S, Yamamoto Y, Okamoto
T, Shibata H, Mukai Y, Taniai M, Shimizu T, Kawamura M, Abe Y, Nakagawa
S, Mayumi T. Selective enhancer of tumor vascular permeability for optimization
of cancer chemotherapy. Biol Pharm Bull., 27:437-439. 2004.[PubMed]
Yoshioka Y, Tsutsumi Y, Mukai Y, Shibata H, Okamoto T, Kaneda Y, Tsunoda
S, Kamada H, Koizumi K, Yamamoto Y, Mu Y, Kodaira H, Sato-Kamada K, Nakagawa
S, Mayumi T. Effective accumulation of poly(vinylpyrrolidone-co-vinyl laurate)
into the spleen. J Biomed Mater Res A., 70:219-223. 2004.[PubMed]
Yamamoto Y, Tsutsumi Y, Yoshioka Y, Kamada H, Sato-Kamada K, Okamoto T,
Mukai Y, Shibata H, Nakagawa S, Mayumi T. Poly(vinylpyrrolidone-co-dimethyl
maleic acid) as a novel renal targeting carrier. J Control Release., 95:229-237.
2004.[PubMed]
Wakita T, Hayashi T, Nishioka J, Tamaru H, Akita N, Asanuma K, Kamda H,
Gabazza EC, Ido M, Kawamura J, Suzuki K. Regulation of carcinoma cell invasion
by protein C inhibitor whose expression is decreased in renal cell carcinoma.
Int J Cancer., 108:516-523. 2004.[PubMed]
Shibata H, Yoshioka Y, Ikemizu S, Kobayashi K, Yamamoto Y, Mukai Y, Okamoto
T, Taniai M, Kawamura M, Abe Y, Nakagawa S, Hayakawa T, Nagata S, Yamagata
Y, Mayumi T, Kamada H, Tsutsumi Y. Functionalization of tumor necrosis
factor-alpha using phage display technique and PEGylation improves its
antitumor therapeutic window. Clin Cancer Res., 10:8293-8300. 2004.[PubMed]
Okamoto T, Mukai Y, Yoshioka Y, Shibata H, Kawamura M, Yamamoto Y, Nakagawa
S, Kamada H, Hayakawa T, Mayumi T, Tsutsumi Y. Optimal construction of
non-immune scFv phage display libraries from mouse bone marrow and spleen
established to select specific scFvs efficiently binding to antigen. Biochem
Biophys Res Commun., 323:583-591. 2004.[PubMed]
Kodaira H, Tsutsumi Y, Yoshioka Y, Kamada H, Kaneda Y, Yamamoto Y, Tsunoda
S, Okamoto T, Mukai Y, Shibata H, Nakagawa S, Mayumi T. The targeting of
anionized polyvinylpyrrolidone to the renal system. Biomaterials., 25:4309-4315.
2004.[PubMed]
Kaneda Y, Tsutsumi Y, Yoshioka Y, Kamada H, Yamamoto Y, Kodaira H, Tsunoda S, Okamoto T, Mukai Y, Shibata H, Nakagawa S, Mayumi T. The use of PVP as a polymeric carrier to improve the plasma half-life of drugs. Biomaterials., 25:3259-3266. 2004.[PubMed]
Kamada H, Tsutsumi Y, Yoshioka Y, Yamamoto Y, Kodaira H, Tsunoda S, Okamoto T,
Mukai Y, Shibata H, Nakagawa S, Mayumi T. Design of a pH-sensitive polymeric
carrier for drug release and its application in cancer therapy. Clin Cancer
Res., 10:2545-2550. 2004.[PubMed]
Kamada H, Hattori K, Hayashi T, Suzuki K. In vitro evaluation of blood coagulation
activation and microthrombus formation by a microchannel array flow analyzer.
Thromb Res., 114:195-203. 2004.[PubMed]
Hayashi T, Nishioka J, Kamada H, Asanuma K, Kondo H, Gabazza EC, Ido M,
Suzuki K. Characterization of a novel human protein C inhibitor (PCI) gene
transgenic mouse useful for studying the role of PCI in physiological and
pathological conditions. J Thromb Haemost., 2:949-961. 2004.[PubMed]
Gabazza EC, Taguchi O, Kamada H, Hayashi T, Adachi Y, Suzuki K. Progress in the understanding of protease-activated receptors. Int J Hematol., 79:117-122. 2004.[PubMed]
永田 諭志
Onda M, Willingham M, Nagata S, Bera TK, Beers R, Ho M, Hassan R, Kreitman
RJ, Pastan I (2005) New monoclonal antibodies to mesothelin useful for
immunohistochemistry, fluorescence-activated cell sorting, Western blotting,
and ELISA. Clin Cancer Res 11, 5840-5846.[PubMed]
Nagata S, Ise T, Onda M, Nakamura K, Ho M, Raubitschek A, Pastan IH (2005)
Cell membrane-specific epitopes on CD30: Potentially superior targets for
immunotherapy. Proc Natl Acad Sci U S A 102, 7946-7951.[PubMed]
Soler-Garcia AA, Maitra R, Kumar V, Ise T, Nagata S, Beers R,
Bera TK, Pastan I (2005) The PATE gene is expressed in the accessory tissues of the human
male genital tract and encodes a secreted sperm-associated protein. Reproduction
129, 515-524.[PubMed]
Bang S, Nagata S, Onda M, Kreitman RJ, Pastan I (2005) HA22 (R490A) is
a recombinant immunotoxin with increased antitumor activity without an
increase in animal toxicity. Clin Cancer Res 11, 1545-1550.[PubMed]
Ise T, Maeda H, Santora K, Xiang L, Kreitman RJ, Pastan I, Nagata S (2005)
Immunoglobulin superfamily receptor translocation associated 2 protein
on lymphoma cell lines and hairy cell leukemia cells detected by novel
monoclonal antibodies. Clin Cancer Res 11, 87-96.[PubMed]
Shibata H, Yoshioka Y, Ikemizu S, Kobayashi K, Yamamoto Y, Mukai Y, Okamoto
T, Taniai M, Kawamura M, Abe Y, Nakagawa S, Hayakawa T, Nagata S, Yamagata
Y, Mayumi T, Kamada H, Tsutsumi Y (2004) Functionalization of tumor necrosis
factor-alpha using phage display technique and PEGylation improves its
antitumor therapeutic window. Clin Cancer Res 10, 8293-8300.[PubMed]
Nagata S, Numata Y, Onda M, Ise T, Hahn Y, Lee B, Pastan I (2004) Rapid
grouping of monoclonal antibodies based on their topographical epitopes
by a label-free competitive immunoassay. J Immunol Methods 292, 141-155.[PubMed]
Maeda H, Nagata S, Wolfgang CD, Bratthauer GL, Bera TK, Pastan I (2004)
The T cell receptor gamma chain alternate reading frame protein (TARP),
a prostate-specific protein localized in mitochondria. J Biol Chem 279,
24561-24568.[PubMed]
Yoshioka Y, Tsutsumi Y, Ikemizu S, Yamamoto Y, Shibata H, Nishibata T,
Mukai Y, Okamoto T, Taniai M, Kawamura M, Abe Y, Nakagawa S, Nagata S,
Yamagata Y, Mayumi T (2004) Optimal site-specific PEGylation of mutant
TNF-alpha improves its antitumor potency. Biochem Biophys Res Commun 315,
808-814.[PubMed]
永田諭志: 抗体の作製 （ポリクローナル抗体とモノクローナル抗体）福井泰久編著 細胞内シグナル伝達研究法. 学会出版センタ－, 2004, pp141-160.
Nagata S, Salvatore G, Pastan I (2003) DNA immunization followed by a single
boost with cells: a protein-free immunization protocol for production of
monoclonal antibodies against the native form of membrane proteins. J Immunol
Methods 280, 59-72.[PubMed]
Yamamoto Y, Tsutsumi Y, Yoshioka Y, Nishibata T, Kobayashi K, Okamoto T,
Mukai Y, Shimizu T, Nakagawa S, Nagata S, Mayumi T (2003) Site-specific
PEGylation of a lysine-deficient TNF-alpha with full bioactivity. Nat Biotechnol
21, 546-552.[PubMed]
Kamada H, Tsutsumi Y, Sato-Kamada K, Yamamoto Y, Yoshioka Y, Okamoto T,
Nakagawa S, Nagata S, Mayumi T (2003) Synthesis of a poly(vinylpyrrolidone-co-dimethyl
maleic anhydride) co-polymer and its application for renal drug targeting.
Nat Biotechnol 21, 399-404.[PubMed]
Kamogae M, Tsutsumi T, Nagata S, Aymoto NM, Ueno Y, Hirooka EY (2002) Monitoring
of Microcystins in Fresh Water for Human Consumption in the Itaipu Dam,
Paran-Brazil. Brazilian Journal of Food Technology 5, 109-116.in press.
Nagata S, Onda M, Numata Y, Santora K, Beers R, Kreitman RJ, Pastan I (2002)
Novel anti-CD30 recombinant immunotoxins containing disulfide-stabilized
Fv fragments. Clin Cancer Res 8, 2345-2355.[PubMed]
Salvatore G, Nagata S, Billaud M, Santoro M, Vecchio G, Pastan I (2002)
Generation and characterization of novel monoclonal antibodies to the Ret
receptor tyrosine kinase. Biochem Biophys Res Commun 294, 813-817.[PubMed]
Shinohara M, Terada Y, Iwamatsu A, Shinohara A, Mochizuki N, Higuchi M,
Gotoh Y, Ihara S, Nagata S, Itoh H, Fukui Y, Jessberger R (2002) SWAP-70
is a guanine-nucleotide-exchange factor that mediates signaling of membrane
ruffling. Nature 416, 759-763.[PubMed]
Weller MG, Zeck A, Eikenberg A, Nagata S, Ueno Y, Niessner R (2001) Development
of a direct competitive microcystin immunoassay of broad specificity. Anal
Sci 17, 1445-8.[PubMed]
Yoshida T, Takeda M, Tsutsumi T, Nagata S, Yoshida F, Maita K, Harada T,
Ueno Y (2001) Tumor Necrosis Factor-alpha Expression and Kupffer Cell Activation
in Hepatotoxicity Caused by Microcystin-LR in Mice. Journal of Toxicologic
Pathology 14, 259-265.in press.
Yoshida T, Tsutsumi T, Nagata S, Yoshida F, Maita K, Harada T, Ueno Y (2001)
Quantitative Analysis of Intralobular Distribution of Microcystin-LR in
the Mouse Liver. Journal of Toxicological Pathology 14, 205-212.in press.
Yoshida T, Nakamura Y, Tsutsumi T, Nagata S, Yoshida F, Maita K, Harada
T, Ueno Y (2001) Transmission Electron and Immunoelectron Microscopic Studies
on Microcystin-LR-Induced Hepatic Injuries in Mice. Journal of Toxicological
Pathology 14, 157-161.in press.
Onda M, Nagata S, Tsutsumi Y, Vincent JJ, Wang Q, Kreitman RJ, Lee B, Pastan
I (2001) Lowering the isoelectric point of the Fv portion of recombinant
immunotoxins leads to decreased nonspecific animal toxicity without affecting
antitumor activity. Cancer Res 61, 5070-5077.[PubMed]
永田諭志: がん治療を目指す組み換えイムノトキシンの開発と臨床応用の現状. 蛋白質 核酸 酵素2001; 46(4 Suppl):540-546.[PubMed]
Onda M, Willingham M, Wang QC, Kreitman RJ, Tsutsumi Y, Nagata S, Pastan
I (2000) Inhibition of TNF-alpha produced by Kupffer cells protects against
the nonspecific liver toxicity of immunotoxin anti-Tac(Fv)-PE38, LMB-2.
J Immunol, 165, 7150-7156.[PubMed]
Nishio Y, Nagata S, Umeda M, Shirai R, Yokogawa T, Ihara S, Fukui Y. (2000)
Quantification of phosphatidylinositol 3,4,5-trisphosphate by liposome
lysis assay with specific monoclonal antibodies. Anal Biochem 285, 270-273.[PubMed]
Tsutsumi T, Nagata S, Yoshida F, Harada KI, Ueno Y (2000) Development and
application of highly sensitive anti-immune complex ELISAs for microcystins
in tap water. Food and Agricultural Immunology 12, 231-241.in press.
Tsutsumi Y, Onda M, Nagata S, Lee B, Kreitman RJ, Pastan I (2000) Site-specific
chemical modification with polyethylene glycol of recombinant immunotoxin
anti-Tac(Fv)-PE38 (LMB-2) improves antitumor activity and reduces animal
toxicity and immunogenicity. Proc Natl Acad Sci U S A 97, 8548-8553.[PubMed]
Yokogawa T, Nagata S, Nishio Y, Tsutsumi T, Ihara S, Shirai R, Morita K,
Umeda M, Shirai Y, Saitoh N, Fukui Y (2000) Evidence that 3'-phosphorylated
polyphosphoinositides are generated at the nuclear surface: use of immunostaining
technique with monoclonal antibodies specific for PI 3,4-P(2). FEBS Lett
473, 222-226.[PubMed]
Tsutsumi T, Nagata S, Hasegawa A, Ueno Y (2000) Immunoaffinity column as
clean-up tool for determination of trace amounts of microcystins in tap
water. Food Chem Toxicol 38, 593-597.[PubMed]
Ueno Y, Makita Y, Nagata S, Tsutsumi T, Yoshida F, Tamura SI, Sekijima
M, Tashiro F, Harada T, Yoshida T (1999) No chronic oral toxicity of a
low dose of microcystin-LR, a cyanobacterial hepatotoxin, in female BALB/c
mice. Environmental Toxicology 14, 45-55.[PubMed]
Nagata S, Tsutsumi T, Yoshida F, Ueno Y (1999) A new type sandwich immunoassay
for microcystin: production of monoclonal antibodies specific to the immune
complex formed by microcystin and an anti-microcystin monoclonal antibody.
Nat Toxins 7, 49-55.[PubMed]
Kobayashi M, Nagata S, Iwasaki T, Yanagihara K, Saitoh I, Karouji Y, Ihara
S, Fukui Y (1999) Dedifferentiation of adenocarcinomas by activation of
phosphatidylinositol 3-kinase. Proc Natl Acad Sci U S A 96, 4874-4879.[PubMed]
Tanaka K, Horiguchi K, Yoshida T, Takeda M, Fujisawa H, Takeuchi K, Umeda
M, Kato S, Ihara S, Nagata S, Fukui Y. (1999) Evidence that a phosphatidylinositol
3,4,5-trisphosphate-binding protein can function in nucleus. J Biol Chem
274, 3919-3922.[PubMed]
Tanaka K, Adachi H, Konishi H, Iwamatsu A, Ohkawa K, Shirai T, Nagata S,
Kikkawa U, Fukui Y (1999) Identification of protein kinase B (PKB) as a
phosphatidylinositol 3,4,5-trisphosphate binding protein in Dictyostelium
discoideum. Biosci Biotechnol Biochem 63, 368-372.[PubMed]
Shirai T, Tanaka K, Terada Y, Sawada T, Shirai R, Hashimoto Y, Nagata S,
Iwamatsu A, Okawa K, Li S, Hattori S, Mano H, Fukui Y (1998) Specific detection
of phosphatidylinositol 3,4,5-trisphosphate binding proteins by the PIP3
analogue beads: an application for rapid purification of the PIP3 binding
proteins. Biochim Biophys Acta 1402, 292-302.[PubMed]
Tsutsumi T, Nagata S, Yoshida F, Ueno Y (1998) Anti-idiotype monoclonal
antibodies against anti-microcystin antibody and their use in enzyme immunoassay.
Toxicon 36, 235-245.[PubMed]
Yoshida T, Makita Y, Tsutsumi T, Nagata S, Tashiro F, Yoshida F, Sekijima
M, Tamura S, Harada T, Maita K, Ueno Y (1998) Immunohistochemical localization
of microcystin-LR in the liver of mice: a study on the pathogenesis of
microcystin-LR-induced hepatotoxicity. Toxicol Pathol 26, 411-418.[PubMed]
Kita Y, Kimura KD, Kobayashi M, Ihara S, Kaibuchi K, Kuroda S, Ui M, Iba
H, Konishi H, Kikkawa U, Nagata S, Fukui Y (1998) Microinjection of activated
phosphatidylinositol-3 kinase induces process outgrowth in rat PC12 cells
through the Rac-JNK signal transduction pathway. J Cell Sci 111, 907-915.[PubMed]
Daduang S, Kimura K, Nagata S, Fukui Y (1998) Density dependent elevation
of phosphatidylinositol-3 kinase level in rat 3Y1 cells. Biochim Biophys
Acta 1401, 113-120.[PubMed]
Ihara S, Nakajima K, Fukada T, Hibi M, Nagata S, Hirano T, Fukui Y (1997)
Dual control of neurite outgrowth by STAT3 and MAP kinase in PC12 cells
stimulated with interleukin-6. EMBO J 16, 5345-5352.[PubMed]
Ichiba T, Kuraishi Y, Sakai O, Nagata S, Groffen J, Kurata T, Hattori S,
Matsuda M. (1997) Enhancement of guanine-nucleotide exchange activity of
C3G for Rap1 by the expression of Crk, CrkL, and Grb2. J Biol Chem 272,
22215-22220.[PubMed]
Yoshida T, Makita Y, Nagata S, Tsutsumi T, Yoshida F, Sekijima M, Tamura
S, Ueno Y. (1997) Acute oral toxicity of microcystin-LR, a cyanobacterial
hepatotoxin, in mice. Nat Toxins 5, 91-95.[PubMed]
Kobayashi M, Nagata S, Kita Y, Nakatsu N, Ihara S, Kaibuchi K, Kuroda S,
Ui M, Iba H, Konishi H, Kikkawa U, Saitoh I, Fukui Y (1997) Expression
of a constitutively active phosphatidylinositol 3-kinase induces process
formation in rat PC12 cells. Use of Cre/loxP recombination system. J Biol
Chem 272, 16089-16092.[PubMed]
Tanaka K, Imajoh-Ohmi S, Sawada T, Shirai R, Hashimoto Y, Iwasaki S, Kaibuchi
K, Kanaho Y, Shirai T, Terada Y, Kimura K, Nagata S, Fukui Y (1997) A target
of phosphatidylinositol 3,4,5-trisphosphate with a zinc finger motif similar
to that of the ADP-ribosylation-factor GTPase-activating protein and two
pleckstrin homology domains. Eur J Biochem 245, 512-519.[PubMed]
Nagata S, Tsutsumi T, Hasegawa A, Yoshida F, Ueno Y, Watanabe MF (1997)
Enzyme Immunoassay for Direct Determination of Microcystins in Environmental
Water. Journal of AOAC International 80, 408-417.in press.
Ueno Y, Nagata S, Tsutsumi T, Hasegawa A, Yoshida F, Suttajit M, Mebs D,
Putsch M, Vasconcelos V (1996) Survey of microcystins in environmental
water by a highly sensitive immunoassay based on monoclonal antibody. Nat
Toxins 4, 271-276.[PubMed]
Kondo F, Matsumoto H, Yamada S, Ishikawa N, Ito E, Nagata S, Ueno Y, Suzuki
M, Harada K (1996) Detection and identification of metabolites of microcystins
formed in vivo in mouse and rat livers. Chem Res Toxicol 9, 1355-1359.[PubMed]
Senpuku H, Iizima T, Yamaguchi Y, Nagata S, Ueno Y, Saito M, Hanada N,
Nisizawa T (1996) Immunogenicity of peptides coupled with multiple T-cell
epitopes of a surface protein antigen of Streptococcus mutants. Immunology
88, 275-283.[PubMed]
Ueno Y, Nagata S, Tsutsumi T, Hasegawa A, Watanabe MF, Park HD, Chen GC,
Chen G, Yu SZ (1996) Detection of microcystins, a blue-green algal hepatotoxin,
in drinking water sampled in Haimen and Fusui, endemic areas of primary
liver cancer in China, by highly sensitive immunoassay. Carcinogenesis
17, 1317-1321.[PubMed]
Ueno Y, Umemori K, Niimi E, Tanuma S, Nagata S, Sugamata M, Ihara T, Sekijima
M, Kawai K, Ueno I (1995) Induction of apoptosis by T-2 toxin and other
natural toxins in HL-60 human promyelotic leukemia cells. Nat Toxins 3,
129-137.[PubMed]
Daduang S, Nagata S, Matsuda M, Yamori T, Onodera K, Fukui Y (1995) Production
of monoclonal antibodies specific to the carboxyl terminal region of the
85 kDa subunit of phosphatidylinositol 3-kinase: use of the antibodies
in recognition of mutant p85. Immunol Cell Biol 73, 134-139.[PubMed]
Nagata S, Soutome H, Tsutsumi T, Hasegawa A, Sekijima M, Sugamata M, Harada
K, Suganuma M, Ueno Y (1995) Novel monoclonal antibodies against microcystin
and their protective activity for hepatotoxicity. Nat Toxins 3, 78-86.[PubMed]
Matsumura S, Ishida T, Washizu T, Tomoda I, Nagata S, Chiba J, Kurata T
(1993) Pathologic features of acquired immunodeficiency-like syndrome in
cats experimentally infected with feline immunodeficiency virus. J Vet
Med Sci 55, 387-394.[PubMed]
Tanaka S, Matsuda M, Nagata S, Kurata T, Nagashima K, Shizawa Y, Fukui
Y (1993) Structure of 85 kDa subunit of human phosphatidylinositol 3-kinase
analyzed by using monoclonal antibodies. Jpn J Cancer Res 84, 279-289.[PubMed]
Matsuda M, Nagata S, Tanaka S, Nagashima K, Kurata T (1993) Structural
requirement of CRK SH2 region for binding to phosphotyrosine-containing
proteins. Evidence from reactivity to monoclonal antibodies. J Biol Chem
286, 4441-4446.in press.
Nagata S, Okamoto Y, Inoue T, Ueno Y, Kurata T, Chiba J (1992) Identification
of epitopes associated with different biological activities on the glycoprotein
of vesicular stomatitis virus by use of monoclonal antibodies. Arch Virol
127, 153-168.[PubMed]
Matsuda M, Tanaka S, Nagata S, Kojima A, Kurata T, Shibuya M (1992) Two
species of human CRK cDNA encode proteins with distinct biological activities.
Mol Cell Biol 12, 3482-3489.[PubMed]
Nagata S, Kurata T, Ueno Y, Chiba J (1991) Vesicular stomatitis virus-mediated
cell fusion subsequent to virus adsorption at different pH values. Jpn
J Med Sci Biol 44, 171-180.[PubMed]
Nagata S, Yamamoto K, Ueno Y, Kurata T, Chiba J (1991) Preferential generation
of monoclonal IgG-producing hybridomas by use of vesicular stomatitis virus-mediated
cell fusion. Hybridoma 10, 369-378.[PubMed]
Nagata S, Yamamoto K, Ueno Y, Kurata T, Chiba J (1991) Production of monoclonal
antibodies by the use of pH-dependent vesicular stomatitis virus-mediated
cell fusion. Hybridoma 10, 317-322.[PubMed]
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